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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E IH

Carcinoembryonic Antigen (CEA) is synthesized during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
Catalog #	C1300-01G
Applications	Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	ELISA: No reaction is observed with normal human plasma stripped of CEA in double antibody sandwich ELISA.
	Immunohistochemistry (Paraffin): 1:75-1:200. Enzymatic antigen retrieval is recommended.
	Optimal dilutions to be determined by the researcher.e
Positive Control	Hepatic metastasis of colon adenocarcinoma
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in 0.1M sodium chloride, pH 7.2, 15mM sodium azide.
Purity	Purified
Immunogen	Human Carcinoembryonic Antigen isolated from hepatic metastasis of colon adenocarcinoma. Cellular Localization: Lumenal membrane
Specificity	Recognizes the human Carcinoembryonic Antigen (CEA). Reacts with CEA like proteins, for example, CEACAM1 and CEACAM6.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.