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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » CEA, BioAssay™ ELISA Kit (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, Carcinoembryonic Antigen, Meconium Antigen 100, CD66e, CEACAM5)

CEA, BioAssay™ ELISA Kit (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5,
Carcinoembryonic Antigen, Meconium Antigen 100, CD66e, CEACAM5)

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Specifications

Carcinoembryonic antigen (CEA) was first described in 1965 by Gold and Freedman when they identified an antigen that was present in both fetal colon and colon adenocarcinoma but that appeared to be absent from healthy adult colon. CEA is a glycoprotein containing approximately 50% carbohydrate with a molecular weight of 180kD. CEA and related genes make up the CEA family belonging to the immunoglobulin superfamily. In humans, the carcinoembryonic antigen family consists of 29 genes, 18 of which are normally expressed.
Catalog #C1300-06A
CEA has proven to be a suitable target antigen for the detection of primary and metastatic colorectal and some other carcinomas. Although sera from healthy individuals and from patients with other diseases generally had low levels of CEA, CEA is one of the most widely used tumor markers worldwide. Its main application is mostly in gastrointestinal cancers, especially in colorectal malignancy. CEA assays are now generally accepted clinically as a useful and cost- efficient tool in monitoring of colon cancer following surgery.
Intended UseHuman Carcinoembryonic Antigen (human CEA) ELISA kit is to be used for the in vitro quantitative determination of human CEA in human serum, human plasma, cell lysate and buffered solution. The assay will recognize native CEA. This kit has been configured for research use only and is not to be used in diagnostic procedures.
SensitivityThe minimal detectable dose of human CEA was calculated to be 0.15ng/ml, by subtracting two standard deviations from the mean of 10 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation.
SpecificityThe following substances were tested and found to have no cross-reactivity: human
Test PrincipleThe design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human CEA. Samples are pippetted into these wells. Nonbound CEA and other components of the sample should be removed by washing, then biotin-conjugated monoclonal antibody specific to CEA added. In order to quantitatively determine the amount of CEA present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured CEA.
Kit ComponentsMicroplate: 1x96 wells
Incubation buffer: 1x30ml
Washing buffer, (10x): 1x100ml
Standard protein: 1 glass vial lyophilized
Standard/sample dilution buffer: 1x25ml
Second antibody: 1 glass vial lyophilized
AV-HRP: 1x150ul
Secondary antibody/AV-HRP dilution buffer: 1x25ml
Substrate (TMB): 1x20ml
Stop solution: 1x20ml
Storage and StabilityStore components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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