| Carcinoembryonic antigen (CEA), which is synthesized during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Anti-CEA is reportedly useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). |
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| Catalog # | C1300-08 |
| Applications | Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunoprecipitation: 10ul/mg protein lysate. Use Protein A, native and denatured. |
| Western Blot: 1:200 for 2hrs at RT |
| Immunohistochemistry (Formalin/paraffin): 1:500-1:1000. Incubate for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissue. |
| Optimal dilution determined by the researcher. |
| Recommended Positive Control | LS174T cells or colon carcinoma |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. |
| Purity | Purified by ammonium sulfate precipitation, followed by ion-exchange chromatography and appropriate solid-phase absorption. |
| Immunogen | Human CEA isolated from hepatic metastasis of colon adenocarcinoma. Cellular Localization |
| Specificity | Recognizes human CEA and CEA-like proteins such as non-specific crossreacting antigen (NCA), NCA2, and biliary glycoprotein (BGP1). Has been absorbed with plasma proteins and blood group antigens A and B. Species Crossreactivity: mouse. Weakly reacts with rat. Does not react with canine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
