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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -Carcinoembryonic Antigen (CEA, CD66e)

Anti -Carcinoembryonic Antigen (CEA, CD66e)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified B IP IH
Carcinoembryonic antigen (CEA), which is synthesized during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Anti-CEA is reportedly useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
Catalog #C1300-08
ApplicationsSuitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunoprecipitation (Native and denatured): 10ul/mg protein lysate. Use Protein A.
Western Blot: 1:200 for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 1:250-1:500. Incubate for 20 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissue.
Optimal dilution determined by the researcher.
Recommended Positive ControlLS174T cells or colon carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
PurityPurified by ammonium sulfate precipitation, followed by ion-exchange chromatography and appropriate solid-phase absorption
ImmunogenHuman CEA isolated from hepatic metastasis of colon adenocarcinoma. Cellular Localization
SpecificityRecognizes human CEA and CEA-like proteins such as non-specific crossreacting antigen (NCA), NCA2, and biliary glycoprotein (BGP1). Has been absorbed with plasma proteins and blood group antigens A and B. Species Crossreactivity: mouse. Weakly reacts with rat. Does not react with canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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