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Specifications

Clone Host Grade Applications Polyclonal Human Purified E

Cardiolipin is an important component of the inner mitochondrial membrane, where it constitutes about 20% of the total lipid composition. The name ‘cardiolipin’ is derived from the fact that it was first found in beef heart in the early 1940s. In mammalian cells, cardiolipin (CL) is found almost exclusively in the inner mitochondrial membrane where it is essential for the optimal function of numerous enzymes that are involved in mitochondrial energy metabolism.
Catalog #	C1375
Applications	Suitable for use in ELISA. Other applications have not been tested.
Recommended Dilutions	ELISA: 1:100
	Optimal dilutions to be determined by the researcher.
In an ELISA system designed to detect all IgG subclasses, the following purified antigens (dilution of 1	100) responded as:
	Antigen Result (EU/ml)
	SSA 33
	SSB 9
	SCL 18
	Sm 7
	Sm/RNP 11
	Jo-1 31
Interpretation of Results	<16 EU/ml: Negative
	16-20 EU/ml: Equivocal
	>20 EU/ml: Positive
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Human
Source	Human
Concentration	Not Determined
Form	Supplied as a lyophilized powder from PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Purity	Purified by delipidation, defibrination and ammonium sulfate precipitation.
Immunogen	Auto-antibody derived from single donor human serum.
Specificity	Recognizes human Cardiolipin (13GPL/ml). This is a weak positive response.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.