| Cardiotin is a high molecular weight protein complex (300kD) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60kD and 100kD, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle alpha-actinin. |
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| Catalog # | C1384-01 |
| During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes. |
| Applications | Suitable for use in Immunohistochemistry (frozen and paraffin sections) and Western Blotting. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry: 1:25-1:100 |
| Western Blot: 1:25-1:500 |
| Optimal dilutions to be determined by researcher. |
| Hybridoma | Derived by fusion of SP2/0-Ag14 mouse myeloma cells, with spleen cells from a mouse. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgM |
| Clone No | 4A17 |
| Host | Mouse |
| Source | Human |
| Concentration | 1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Cardiotin |
| Specificity | Reacts with a 300kD protein complex and its 100kD and 60kD subunits in immunoblotting assays. Reacts exclusively with cardiomyocytes. Species crossreactivity: Human, monkey, porcine, feline, canine, goat, hamster, mouse, Xenopus. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
