| Cardiotrophin-1 (CT-1) is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT-1 was also detected in abundance in normal adult human lungs and was expressed in both fetal and adult airway smooth muscle cells. |
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| Catalog # | C1385-12 |
| CT-1 activates gp130-dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes. CT-1 also has a neurotrophic function. CT-1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during the period between embryonic day 14 and the first postnatal week. |
| Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. CT-1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. CT-1 can induce expression of the protective heat shock proteins (hsps) in cardiac cells. CT-1 increases ventricular expression of ANP, brain natriuretic peptide (BNP) and angiotensinogen mRNA. CT-1 levels are significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction. |
| Applications | Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. |
| Recommended Dilution | ELISA: To detect hCT-1 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant hCT-1. |
| Western Blot: To detect hCT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCT-1 is 1.5-3.0ng/lane, under reducing or non-reducing conditions. |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hCT-1 (15ng/ml), a concentration of 0.25-0.40ug/ml of this antibody is required. |
| Optimal dilutions to be determined by the researcher. |
| Reconstitution | Reconstitute to 0.2mg/ml with sterile dH2O or sterile 40-50% glycerol for long term storage. |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Country of Origin | USA |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Highly pure (> 98%) recombinant human Cardiotrophin-1 |
| Specificity | Recognizes human Cardiotrophin-1. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
