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You are here:Home » Antibodies » Abs to Neurotrophins » Anti -Cardiotrophin 1 (CT-1)

Anti -Cardiotrophin 1 (CT-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
Cardiotrophin-1 (CT-1) is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT-1 was also detected in abundance in normal adult human lungs and was expressed in both fetal and adult airway smooth muscle cells.
Catalog #C1385-12
CT-1 activates gp130-dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes. CT-1 also has a neurotrophic function. CT-1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during the period between embryonic day 14 and the first postnatal week.
Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. CT-1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. CT-1 can induce expression of the protective heat shock proteins (hsps) in cardiac cells. CT-1 increases ventricular expression of ANP, brain natriuretic peptide (BNP) and angiotensinogen mRNA. CT-1 levels are significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.
ApplicationsSuitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.
Recommended DilutionELISA: To detect hCT-1 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant hCT-1.
Western Blot: To detect hCT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCT-1 is 1.5-3.0ng/lane, under reducing or non-reducing conditions.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hCT-1 (15ng/ml), a concentration of 0.25-0.40ug/ml of this antibody is required.
Optimal dilutions to be determined by the researcher.
ReconstitutionReconstitute to 0.2mg/ml with sterile dH2O or sterile 40-50% glycerol for long term storage.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly pure (> 98%) recombinant human Cardiotrophin-1
SpecificityRecognizes human Cardiotrophin-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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