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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

This protein belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10
Catalog #	C1385-26B
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:32,000.
	Western Blot: 1-3ug/ml. ~ 150kD band observed in lysates of cell line Jurkat (calculated MW of 133kD according to NP_115791.2).
	Optimal dilutions to be determined by the researcher.
Storage and Stability
	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide: QRKTIWVDEDQL representing the C-Terminus of the human protein (residues 1131-1147), according to NP_115791.
Specificity	Species Crossreactivity: Human. Expected to crossreact with Mouse due to sequence homology.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	BIMP3, Caspase Recruitment Domain Family Member 11