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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -CARM1 (CARM-1, Coactivator-Associated Arginine Methyltransferase 1, PRMT4, Protein Arginine N-methyltransferase 4)

Anti -CARM1 (CARM-1, Coactivator-Associated Arginine Methyltransferase 1, PRMT4, Protein
Arginine N-methyltransferase 4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. CARM1 (coactivator-associated arginine methyltransferase-1) also known as PRMT4 (protein-arginine N-methyltransferase-4) belongs to a family of proteins that catalyzes the methylation of arginine residues. The PRMT proteins are recruited by C-terminal activation domain-2 of the SRC (steroid receptor coactivator) GRIP-1, whereas p300 interacts with activation domain-1 in the C terminus. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduces both methyltransferase and coactivator activities suggesting that coactivator-mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.
Catalog #C1386-31F
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.5-4 ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide C1386-31F1
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
PurityPurified by immunoaffinitiy chromatography.
ImmunogenSynthetic peptide surrounding aa569 of human CARM1.
SpecificityRecognizes human CARM1at -63kD. Species crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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