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You are here:Home » Antibodies » Abs to Drugs » Anti -CART (Cocaine and Amphetamine Related Transcript, CARP)

Anti -CART (Cocaine and Amphetamine Related Transcript, CARP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Cocaine- and Amphetamine Related Transcript (CART) was initially identified using PCR differential display as mRNA whose levels in the brain was specifically induced by psychomotor stimulants such as cocaine and amphetamine. CART encodes a secretory single chain polypeptide of 129aa or 116aa protein due to the usage of alternate splicing and processing. The first 27aa represents the hydrophobic core indicative of signal peptide. The CART protein also has several basic amino acids near the 54-66 residues with a potential to form multiple proteolytic forms. CART mRNA was specially enriched in the hypothalamus. In the striatum, CART expression was induced 4-5 fold by cocaine and amphetamine. CART is highly conserved between human and mouse (95% amino acid homology); CART gene was localized to human chromosome Most recently, CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus. Obese animals have virtually no CART. Peripheral administration of leptin in obese animals induces CART. Intracereberoventricular injection of recombinant CART inhibits both normal and starvation-induce feeding, and completely inhibits NPY-induce feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake. CART 55-102 (numbers corresponds to the predicted signal-peptide-cleavage site in the long form of CART) was physiologically active in inhibiting feeding in normal rats.
Catalog #C1386-50D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000
ELISA: 1:10,000-1:50,000, use 5-10ug control peptide/1ul
Optimal dilutions to be determined by the researcher.
Control Peptide C1386-50A
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
PuritySerum
ImmunogenMouse/Rat CART (55-102) peptide
Specificity100% conserved in mouse, rat, sheep and 97% in human CART.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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