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You are here:Home » Antibodies » Abs to Drugs » Anti -CART (Cocaine and Amphetamine Related Transcript, CARP)

Anti -CART (Cocaine and Amphetamine Related Transcript, CARP)

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Serum E B
Cocaine- and Amphetamine Related Transcript (CART) was initially identified using PCR differential display as mRNA whose levels in the brain was specifically induced by psychomotor stimulants such as cocaine and amphetamine (1). CART encodes a secretory single chain polypeptide of 129aa or 116aa protein due to the usage of alternate splicing and processing. The first 27aa represents the hydrophobic core indicative of signal peptide. The CART protein also has several basic amino acids near the 54-66 residues with a potential to form multiple proteolytic forms. CART mRNA was specially enriched in the hypothalamus. In the striatum, CART expression was induced 4-5 fold by cocaine and amphetamine. CART is highly conserved between human and mouse (95% amino acid homology); CART gene was localized to human chromosome 5 (2).
Catalog #C1386-55K
Most recently, CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus (3). Obese animals have virtually no CART. Peripheral administration of leptin in obese animals induces CART. Intracereberoventricular injection of recombinant CART inhibits both normal and starvation-induce feeding, and completely inhibits NPY-induce feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake. CART 55-102 (numbers corresponds to the predicted signal-peptide-cleavage site in the long form of CART) was physiologically active in inhibiting feeding in normal rats (3).
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: 1:10,000-50,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceMouse
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 40% glycerol.
PuritySerum
ImmunogenMouse/Rat CART (55-102) peptide
SpecificityThe peptide is 100% conserved in mouse and rat. It has only 1 conservative change in human CART (47/48 AA; 97%). Partial sequence reported from sheep CART is also 100% conserved. Antibody crossreactivity in various species is not known
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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