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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Cartilage Intermediate-Layer Protein 1 (CILP-1)

Anti -Cartilage Intermediate-Layer Protein 1 (CILP-1)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B
The CILP-1 (cartilage intermediate-layer protein 1) gene product is a monomeric glycoprotein precursor of two secreted, proteolytically generated products, a 90kD N-terminal CILP-1, and a 62kD C-terminal NTPPHase-homolog. It is found in both hyaline and fibrocartilage. Although the C-terminal NTPPHase-homolog (aa 725-1184) does not show enzyme activity, it may participate in matrix structural integrity. Human CILP-1 C-terminus shares 93% and 94% aa identity with porcine and mouse CILP-1 C-terminus, respectively.
Catalog #C1394-50
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml detection limit for rhCILP-1 is ~ 1.3ng/well.
Western Blot: 0.1-0.2ug/ml detection limit for rhCILP-1 is ~ 20ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived rhCILP-1. (aa 724 - 1184)
SpecificityRecognizes human CILP-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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