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Anti -Cartilage Intermediate-Layer Protein 1 (CILP-1)
Anti -Cartilage Intermediate-Layer Protein 1 (CILP-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B |
|
| The CILP-1 (cartilage intermediate-layer protein 1) gene product is a monomeric glycoprotein precursor of two secreted, proteolytically generated products, a 90kD N-terminal CILP-1, and a 62kD C-terminal NTPPHase-homolog. It is found in both hyaline and fibrocartilage. Although the C-terminal NTPPHase-homolog (aa 725-1184) does not show enzyme activity, it may participate in matrix structural integrity. Human CILP-1 C-terminus shares 93% and 94% aa identity with porcine and mouse CILP-1 C-terminus, respectively. | | | Catalog # | C1394-50 | | Applications | Suitable for use in Direct ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Direct ELISA: 0.5-1.0ug/ml detection limit for rhCILP-1 is ~ 1.3ng/well. | | Western Blot: 0.1-0.2ug/ml detection limit for rhCILP-1 is ~ 20ng/lane under non-reducing and reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | NS0-derived rhCILP-1. (aa 724 - 1184) | | Specificity | Recognizes human CILP-1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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