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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -Catenin, p120, CT

Anti -Catenin, p120, CT

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP IF
Originally characterized as a prominent substrate for Src (14) and receptor protein tyrosine kinases (eg, EGF, PDGF, CSF-1) (6), p120ctn is a novel catenin (10, 13) which binds directly to the juxta- membrane region of classical cadherins (eg, E-cadherin)(3 ,11,13,18). Emerging evidence suggests a prominent role in the regulation of cadherin mediated adhesion (1,4,12). Most cells express multiple isoforms of p120 that arise by alternative splicing (8, 9, 11), some of which can be distinguished using monoclonal antibodies (17). Fibroblasts express mostly type 1 isoforms containing an N-terminal extension of 101 amino acids. Type I isoforms may be important for cell motility and are selectively recognized. Epithelial cells preferentially express shorter isoforms lacking this extension. Heterogeneous or negative p120 staining has been reported in breast (5) and colon carcinomas (7, 16) and decreased p120 expression in bladder tumors correlated with a poor prognosis (15). Loss of p120 or E-cadherin never occurs in epithelial tissues under normal circumstances, and its expression is probably mandatory for normal tissue architecture. Like its cousin b-catenin, p120 may have dual roles in both cell-cell adhesion and in nuclear signaling.
Catalog #C2069-39
ApplicationsSuitable for use in Immunofluorescence (canine), ELISA (immunogen), Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:250-1:500
ELISA: 1:1000-1:10,000
Western Blot: 1:1000
Immunoprecipitation (Native): 5-10ul/IP reaction
Optimal dilutions to be determined by the researcher.
Positive ControlMDCK cells (canine), NIH 3T3 cells (mouse), Va-2 broblasts (human), HeLa cells (human), and rat-2 broblasts.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3G58
HostMouse
ConcentrationNot determined
FormSupplied as a liquid, 0.05% sodium azide.
PurityAscites
ImmunogenRecombinant fusion protein derived from the C-terminus of murine p120 Catenin
SpecificityRecognizes p120 Catenin proteins and can be used to detect isoforms 1-4 of the protein. Reacts with a C-terminal epitope found in all known p120 isoforms.
Species CrossreactivityHuman, mouse, rat and canine. Reactivity with the chicken protein is weak but detectable.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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