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You are here:Home » Antibodies » Abs to Catenin » Anti -Catenin, alpha T (a-T-Catenin, Catenin alpha-3)

Anti -Catenin, alpha T (a-T-Catenin, Catenin alpha-3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IC IF
Catenins are proteins that connect cadherin/beta-catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, alpha-T-catenin is expressed in other tissues, the highest levels being observed in the heart. Immunohistochemical analysis showed human alpha-T-catenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that alpha-T-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking alpha-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells.
The encoded protein has about the same predicted size (100kD) as other alpha-catenins to which it shows an overall amino acid identity of 57%. In cells transfected with alpha-T-catenin cDNA, interaction with beta-catenin was demonstrated by co-immunoprecipitation.
Catalog #C2069-41A
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: Acetone-fixed cells
Immunocytochemistry: Methanol and paraformaldehyde-fixed cells, followed by permeabilization in 0.2% Triton X-100 for 2 min.
Immunohistochemistry: 1:12.5-1:100; Avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Western Blot: 1:50-1:500
Optimal dilutions to be determined by the researcher.
HybridomaDerived from a fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a C57BI/6 mouse.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No4A21
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa164-177 of the human alpha T Catenin protein including an additional N-terminal cysteine, coupled to KLH [C-VANKSDLQKTYQKL]
SpecificityRecognizes human alpha T Catenin. Epitope is located between residues 164-177. Does not crossreact with alpha E Catenin or alpha N Catenin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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