Anti -Catenin alpha1 (CTNNA1, Catenin (Cadherin-associated Protein), alpha 1, 102kDa,
HGNC:2509, CAP102, FLJ36832, alpha 1 (102kD), alpha-E-Catenin, alpha-Catenin,
alphaE-Catenin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IP IH IF |
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| a-catenin is a 102kD protein which is related to the cytoskeletal protein vinculin. Vinculin associates with both cell-cell and cell-extracellular matrix adherens-type junctions, and has been shown to interact with both a-actinin and actin. The homology between a-catenin and vinculin is localized to three major regions (amino-terminal, central, and carboxy-terminal portions) with the c-termini exhibiting the highest overall homology . Interestingly, it is the carboxy terminal domain of vinculin which is responsible for its self-association properties . The homology between the c-termini of catenin and vinculin led to speculation that a-catenin can self-associate and/or co-associate with vinculin thereby linking the cadherin/catenin complex to the cytoskeleton. However, recent studies indicate that cadherin/catenin complexes are linked to the actin cytoskeleton via a direct association between a-actinin and a-catenin. In addition, the a-catenin protein has been found to posses both actin binding and bundling activities. | | | Catalog # | C2069-43 | | Applications | Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 2-5ug/ml | | ELISA: 0.1-1ug/ml | | Western Blot: 1ug/ml | | Immunohistochemistry ( formalin- xed, paraf n-embedded tissue): 2-10ug/ml. Heat-Induced Epitope Retrieval (HIER) is required. Expected staining pattern: Membrane with limited cytoplasmic. | | Immunoprecipitation: 5ug | | Optimal dilutions to be determined by the researcher. | | Positive Control | Pancreas/breast tumor/heart | | Source | Ascites | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 3G59 (7A4) | | Host | Mouse | | Source | Mouse | | Concentration | 0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Also available diluted at 1ml. See C2069-43E. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide derived from the C-terminus of mouse a-catenin. | | Specificity | Recognizes mouse a-catenin. Does not crossreact with the related beta- and gamma-catenin proteins. Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Catenin alpha1 (CTNNA1, Catenin (Cadherin-associated Protein), alpha 1, 102kDa, HGNC:2509, CAP102, FLJ36832, alpha 1 (102kD), alpha-E-Catenin, alpha-Catenin, alphaE-Catenin) |
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