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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP

Adherens junctions (AJ) (also referred to as zonula adherens) are required for both the establishment and maintenance of epithelial layers (2). In addition, these junctions have been identi ed in several other cell types including cardiac myocytes and broblasts (2). AJ subserve several important functions including: mediating intercellular adhesion, sensing the presence of neighboring cells, and anchoring the actin cytoskeleton (2,3). AJ are multiprotein complexes that are assembled around cell adhesion molecules called cadherins. Cadherins are a multifunctional family of Ca2+-dependent, transmembrane, glycoproteins which promote cell-cell adhesion (4). The cadherin extracellular domain mediates homophilic interactions between like cadherin molecules on neighboring cells, while the intracellular domain interacts with several cytoplasmic proteins which include: a-catenin, b-catenin, g-catenin (plakoglobin), and the tyrosine kinase substrate p120 cas (1,5,6,7,8,9). Cadherin-catenin interactions are required for complete cadherin activity and regulate the interaction between cadherins and the actin-based cytoskeleton (1,4,6,8,9,10). In fact, deletion of the cadherin cytoplasmic domain produces an adhesion-defective molecule that is unable to interact with the cytoskeleton (4,11).
Catalog #	C2069-44
Applications	Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	ELISA: 0.1-1ug/ml
	Western Blot: 1-3ug/ml
	Immunoprecipitation: 5-15ug
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide derived from the C-terminus of mouse a-Catenin.
Specificity	Speci c for a-catenin, a 102kD protein. Does not crossreact with the related b-catenin or g-catenin proteins.
Species Crossreactivity	Human, Mouse, Rat, Chicken, Xenopus. Tissues/Lysates Tested: HeLa cells, A431 cells, WI-38 human broblasts, Xenopus Cytosol, NIH 3T3 cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.