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You are here:Home » Antibodies » Abs to Catenin » Anti -a-Catenin

Anti -a-Catenin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP
Adherens junctions (AJ) (also referred to as zonula adherens) are required for both the establishment and maintenance of epithelial layers (2). In addition, these junctions have been identi ed in several other cell types including cardiac myocytes and broblasts (2). AJ subserve several important functions including: mediating intercellular adhesion, sensing the presence of neighboring cells, and anchoring the actin cytoskeleton (2,3). AJ are multiprotein complexes that are assembled around cell adhesion molecules called cadherins. Cadherins are a multifunctional family of Ca2+-dependent, transmembrane, glycoproteins which promote cell-cell adhesion (4). The cadherin extracellular domain mediates homophilic interactions between like cadherin molecules on neighboring cells, while the intracellular domain interacts with several cytoplasmic proteins which include: a-catenin, b-catenin, g-catenin (plakoglobin), and the tyrosine kinase substrate p120 cas (1,5,6,7,8,9). Cadherin-catenin interactions are required for complete cadherin activity and regulate the interaction between cadherins and the actin-based cytoskeleton (1,4,6,8,9,10). In fact, deletion of the cadherin cytoplasmic domain produces an adhesion-defective molecule that is unable to interact with the cytoskeleton (4,11).
Catalog #C2069-44
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunoprecipitation: 5-15ug
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the C-terminus of mouse a-Catenin.
SpecificitySpeci c for a-catenin, a 102kD protein. Does not crossreact with the related b-catenin or g-catenin proteins.
Species CrossreactivityHuman, Mouse, Rat, Chicken, Xenopus. Tissues/Lysates Tested: HeLa cells, A431 cells, WI-38 human broblasts, Xenopus Cytosol, NIH 3T3 cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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