| Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. alpha-catenin can bind to beta-catenin and can also bind actin. beta-catenin binds the cytoplasmic domain of some cadherins. When beta-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in beta-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When beta-catenin is not associated with cadherins and alpha-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that beta-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer. |
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| Applications: |
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| Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry, Flow Cytometry and Western Blot. Other applications not tested. |
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| Recommended Dilution: |
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| Immunohistochemisty (Paraffin): Detects b-Catenin in mouse embryo sections. |
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| Immunocytochemistry: Shows positive staining in LiCl-treated 293T cells fixed with methanol. |
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| Western Blot: 0.2-2ug/ml; A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C2069-47. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. |
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| Flow Cytometry: 1ug/ml |
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| Optimal dilutions to be determined by the researcher. |
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| Positive Antigen Control Included: |
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| A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. |
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| Storage and Stability: |
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| May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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| Catalog # | C2069-47 |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 3C196 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 15mM sodium chloride, 0.05% sodium azide. Also available without sodium azide. See C2069-47A. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Fusion protein corresponding to residues 1-100 of human beta-catenin with a C-terminal histidine tag. Epitope: Corresponds to aa36-44 (HSGATTTAP) of human beta-catenin, Mr 92kD. |
| Specificity | Recognizes the active form of human beta-catenin, dephosphorylated on Ser37 and Thr41. Non-specific proteins, of MW 65kD and 148kD, were also detected. Species Crossreactivity |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
