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You are here:Home » Antibodies » Abs to Catenin » Anti -Catenin, beta, active (ABC) (BSA, Azide & Glycerol Free)

Anti -Catenin, beta, active (ABC) (BSA, Azide & Glycerol Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IC
Catalog #C2069-47A
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects beta-catenin in RIPA lysates from A431 cells.
Immunohistochemisty: Detects b-catenin in mouse embryo sections.
Immunocytochemistry: Detects b-catenin in LiCl-treated 293Tcells fixed iwth methanol.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3C196
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4 No stabilizing proteins added. No preservative added. Also available w/ azide. See C2069-47.
PurityPurified by Protein G affinity chromatography.
ImmunogenFusion protein corresponding to residues 1-100 of human beta-catenin with a C-terminal histidine tag. Epitope: Corresponds to aa 36-44 (HSGATTTAP) of human beta-catenin, Mr 92kD.
SpecificitySpecific for the active form of beta-catenin, non-phosphorylated on Ser37 and Thr41.1 A non-specific protein, Mr 130kD, was also detected when used at higher concentrations. Species Crossreactivity: Mouse and rat (100%). Predicted to crossreact with frog and chicken (97/100), porcine (99/100) and zebrafish (91/100) based on epitope sequence homology. Does not crossreact with Drosophila. Others not tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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