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Anti -Catenin, beta, active (ABC) (BSA, Azide & Glycerol Free)
Anti -Catenin, beta, active (ABC) (BSA, Azide & Glycerol Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH IC |
|
| Catalog # | C2069-47A | | Applications | Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-2ug/ml detects beta-catenin in RIPA lysates from A431 cells. | | Immunohistochemisty: Detects b-catenin in mouse embryo sections. | | Immunocytochemistry: Detects b-catenin in LiCl-treated 293Tcells fixed iwth methanol. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 3C196 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4 No stabilizing proteins added. No preservative added. Also available w/ azide. See C2069-47. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Fusion protein corresponding to residues 1-100 of human beta-catenin with a C-terminal histidine tag. Epitope: Corresponds to aa 36-44 (HSGATTTAP) of human beta-catenin, Mr 92kD. | | Specificity | Specific for the active form of beta-catenin, non-phosphorylated on Ser37 and Thr41.1 A non-specific protein, Mr 130kD, was also detected when used at higher concentrations. Species Crossreactivity: Mouse and rat (100%). Predicted to crossreact with frog and chicken (97/100), porcine (99/100) and zebrafish (91/100) based on epitope sequence homology. Does not crossreact with Drosophila. Others not tested. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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