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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH IC

ß-catenin is a key regulatory protein involved in cell adhesion and signal transduction through the Wnt pathway, and plays important roles in development, cellular proliferation, and differentiation (1). ß-catenin is phosphorylated at four serine and threonine residues at the N-terminus (2). Ser 45 is phosphorylated by CK1, and is thought to be a priming site for the phosphorylation by glycogen synthase kinase-3 (GSK-3) of the remaining three residues, Thr 41, Ser 37, and Ser 33 (2). Wnt signaling antagonizes the action of GSK-3, which in subsequent phosphorylation steps, leads to its degradation (3). Mutations in the ß-catenin gene, CTNNB1, have been implicated in its accumulation and various forms of carcinomas (4).
Catalog #	C2069-47B
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blotting: 1:5,000-10,000
	Immunohistochemistry: 1:500
	Immunocytochemistry: 1:250
	Immunoprecipitation: 1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	6k69
Host	Rabbit
Source	Human
Form	Supplied as a liquid
Purity	Supernatant
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.