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You are here:Home » Antibodies » Abs to Catenin » Anti -Catenin, beta

Anti -Catenin, beta

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IC
The alpha-, beta- and gamma-catenins are cytoplasmic proteins mediating the interaction of Ca2+ -dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of beta-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighboring cells. Recent studies indicate that beta-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. Beta-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that beta-catenin plays a dual role not only in the maintenance and regulation of cell-cell interactions but also in the regulation of gene activity.
Catalog #C2069-48C
ApplicationsSuitable for use in ELISA, Western Blot, and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5ug/ml
Immunocytochemistry: 1-10ug/ml
ELISA: 0.05ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control (supplied)Cell lysate of 2x10e6 LiCl treated SW480 cells. Supplied as a lyophilized powder. Reconstitute with 200ul sterile, ddH2O.
Storage and StabilityLyophilized powder may be stored at -20°C for short-term only.Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9L80
HostMouse
ConcentrationAs reported
FormSupplied as a lyophilized powder from 2X PBS, PEG, sucrose, 0.1% sodium azide. Reconstitute with 1ml sterile dH2O.
PurityPurified by thiophilic adsorption and size exclusion chromatography.
ImmunogenSynthetic peptide corresponding to Catenin, beta.
SpecificityRecognizes beta-catenin. Species Crossreactivity: human, mouse, dog.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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