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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IC

The alpha-, beta- and gamma-catenins are cytoplasmic proteins mediating the interaction of Ca2+ -dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of beta-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighboring cells. Recent studies indicate that beta-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. Beta-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that beta-catenin plays a dual role not only in the maintenance and regulation of cell-cell interactions but also in the regulation of gene activity.
Catalog #	C2069-48C
Applications	Suitable for use in ELISA, Western Blot, and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 0.5ug/ml
	Immunocytochemistry: 1-10ug/ml
	ELISA: 0.05ug/ml
	Optimal dilutions to be determined by the researcher.
Positive Control (supplied)	Cell lysate of 2x10e6 LiCl treated SW480 cells. Supplied as a lyophilized powder. Reconstitute with 200ul sterile, ddH2O.
Storage and Stability	Lyophilized powder may be stored at -20°C for short-term only.Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	9L80
Host	Mouse
Concentration	As reported
Form	Supplied as a lyophilized powder from 2X PBS, PEG, sucrose, 0.1% sodium azide. Reconstitute with 1ml sterile dH2O.
Purity	Purified by thiophilic adsorption and size exclusion chromatography.
Immunogen	Synthetic peptide corresponding to Catenin, beta.
Specificity	Recognizes beta-catenin. Species Crossreactivity: human, mouse, dog.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.