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You are here:Home » Antibodies » Abs to Catenin » Anti -Catenin, beta

Anti -Catenin, beta

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP IH IC
Catenins are cytosolic proteins found in varying abundance in many developing and adult tissues (2). Plakoglobin and beta-catenin (95kD) are homologous proteins and can subsitute for one another in the cadherin/catenin complex (2). Their activities are controlled by three types of interactions: those with cadherins in adherens junctions, linking them to the actin cytoskeleton; interactions in the nucleus, where they bind to transcription factors and stimulate gene expression; interactions of free cytoplasmic beta-catenin with axin and adenomatous polyposis coli (APC) protein which target it for degradation. Studies in the past year have demonstrated the complex interplay between these three types of interactions and the different behavior of beta-catenin and plakoglobin. Their involvement in morphogenesis and tumorigenesis strongly suggests that catenins play key roles in adhesion-mediated signaling. beta-Catenin links cadherin cell adhesion molecules to the cytoskeleton and has recently been implicated in the wnt/wingless signaling pathway and in the progression of neoplasia. beta-Catenin associates with the tumor suppressor adenomatous polypopsis coli (APC) and functions as a co-activator of the TCF transcription factor family. It is regulated by glycogen synthase kinase 3b (GSK 3b), which phosphorylates and targets beta-Catenin for ubiquitin-mediated proteolysis. Stabilization of beta-catenin, either by wnt-mediated inhibition of GSK3b or by mutation (as found in numerous colorectal tumors and melanoma cell lines), results in activation of TCF responsive genes which are thought to play key roles in development and cancer progression.
Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and ELISA. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Formalin-fixed paraffin-embedded tissues): 1:250-1:500. Requires pressure cooker antigen retrieval method (*See attached protocol).
Western Blot: 1:500. Detects a band at ~94kD using 10ug of L6 lysates.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalog #C2069-49
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid. No preservative added.
PuritySerum
ImmunogenRecombinant GST-fusion protein (full length beta-catenin).
SpecificityRecognizes human beta Catenin. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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