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Anti -Catenin, beta, phosphorylated (Thr41/Ser45) (Beta Catenin, Cadherin-associated Protein, Catenin beta 1, CATNB, CTNNB, CTNNB1)
Anti -Catenin, beta, phosphorylated (Thr41/Ser45) (Beta Catenin, Cadherin-associated
Protein, Catenin beta 1, CATNB, CTNNB, CTNNB1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| b-Catenin is a 92kD protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of b-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between a- and b-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of b-Catenin. Src family kinases can also phosphorylate tyrosine 654 in the C-terminal armadillo repeat of b-Catenin. This phosphorylation regulates b-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of b-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion. | | | Catalog # | C2069-49R | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:500-1:1000 | | Optimal dilutions to be determined by the researcher. | | Positive Control | SW626 cell | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic phosphopeptide corresponding to human b-Catenin around the phosphorylation site of threonine 41/serine 45 (A-T-TP-T-A-P-SP-L-S). | | Specificity | Recognizes human b-Catenin, phosphorylated at Thr41/Ser45. Species Crossreactivity: mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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