| The catenins (a, b and g) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. b-catenin also binds to N-cadherin and coimmunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between a-actinin and a-catenin. Increased tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion. |
|
| Catalog # | C2069-50AX |
| Applications | Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications were not tested. |
| Recommended Dilutions | Immunoprecipitation (Native verified): 10ug/mg protein lysate using Protein A. |
| Western Blot: 2.5-5ug/ml for 2hrs at RT. |
| Immunohistochemistry (Formalin/paraffin): 10-20 ug/ml for 30 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. |
| Optimal dilutions to be determined by researcher. |
| Positive Control | MCF-7 cells. Breast carcinoma. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C2069-50A. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Recombinant human Beta-catenin protein. MW of Antigen: 92kD |
| Cellular Localization | Cytoplasmic and cell membrane |
| Specificity | Recognizes human Catenin, beta. Species Crossreactivity: mouse. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
