Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Catenin » Anti -Catenin, beta

Anti -Catenin, beta

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
The catenins (a, b and g) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. b-catenin also binds to N-cadherin and coimmunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between a-actinin and a-catenin. Increased tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.
Catalog #C2069-50B
ApplicationsSuitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoprecipitation (Native verified): 10ug/mg Use Protein A.
Western Blot: 5-10ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlMCF-7 cells. Breast carcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2069-50BX.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide from the C-terminus of the Beta-catenin protein. Cellular Localization: Cytoplasmic and cell membrane
SpecificityRecognizes human Catenin, beta at ~92kD.
Species CrossreactivityMouse, rat, chicken and Xenopus
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links