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You are here:Home » Antibodies » Abs to Catenin » Anti -Catenin, beta, phosphorylated (Ser675)

Anti -Catenin, beta, phosphorylated (Ser675)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit B IP IF
B-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates B-catenin on Ser45. This phosphorylation event primes B-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3B destabilizes B-catenin by phosphorylating it at Ser33, Ser37 and Thr41 (7). Mutations in these phosphorylation sites, which result in the stabilization of B-catenin protein levels, have been found in many tumor cell lines (8). PKA was shown to phosphorylate B-catenin at Ser675. Phosphorylation at Ser675 induces B-catenin accumulation in the nucleus and increases its transcriptional activity (9,10).
Catalog #C2069-50F
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunofluorescence (IF-IC). Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation 1:200.
Immunofluorescence (IF-IC) 1:100.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Isotype
Clone No10E12
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied in 10 mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Ser675 of human B-catenin (KLH-coupled).
SpecificityRecognizes human Phospho-B-Catenin (D2F1). Sequence Homology: mouse, rat, chicken, xenopus, zebrafish.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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