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You are here:Home » Antibodies » Antibodies-Catenin » Anti -Catenin, beta, Core

Anti -Catenin, beta, Core


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Clone Host Grade Applications
Monoclonal Mouse Purified E B IP IC
The alpha-, beta- and gamma-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of b-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighbouring cells. Recent studies indicate that b-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. Beta-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that b-catenin plays a dual role not only in the maintainance and regulation of cell-cell interactions but also in the regulation of gene activity.
Catalog #C2069-52C
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionELISA: 0.05ug/ml
Western Blot: 0.5ug/ml for HRP/ECL detection. Recommended blocking buffer: Casein/Tween-20 based blocking buffer.
Immunocytochemistry: 0.1-1ug/ml
Immunoprecipitation: 1-10ug/ml per 10e6 vanadate treated A431cells.
Optimal dilutions to be determined by the researcher.
Positive Antigen Control (Included)C2069-52C1: Reconstitue in SDS-PAGE sample buffer and incubate at 90ºC for 5 minutes. Recommended for Western Blot at 20ul/lane.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No6D206
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.1% sodium azide, PEG, sucrose.
PurityPurifed from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
ImmunogenRecombinant b-catenin.
SpecificityRecognizes the core region (armadillo repeats) of human, mouse and canine b-catenin. Detects a band of ~90kD by Western Blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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