| ß-Catenin is a 92kD protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of ECadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of ß-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine |
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| 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between a- and ß-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of ß-Catenin. Src family kinases can also phosphorylate tyrosine 654 in the |
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| C-terminal armadillo repeat of ß-Catenin. This phosphorylation regulates ß-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of ß-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion. |
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| Catalog # | C2069-54E |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation or for antigen applications in immunological protocols. Other applications not tested. |
| Recommended Dilution | Western blotting 1:1000. Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween 20 for 1hour at room temperature. |
| Immunoprecipitation: 5-10ul |
| ELISA: 1:2000 |
| Optimal dilutions to be determined by the researcher. |
| Control Peptide | C2069-54A Catenin, beta, Control Peptide |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | ß-Catenin synthetic peptide (coupled to carrier protein) corresponding to amino acid residues in the N-terminal region of human ß-Catenin. This human sequence is highly conserved in rat and mouse ß-Catenins. |
| Specificity | Detects a 92kD protein corresponding to the molecular mass of ß-Catenin on SDS-PAGE immunoblots of Hct116 src transformed cells. Similar results were seen in human endothelial, A431, and HeLa cells. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
