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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IF

Plakoglobin (gamma-catenin) is an 83kD protein that localizes to the cytoplasmic face of both desmosomal and adherens junctions. (4) Plakoglobin was rst demonstrated to associate with the desmosomal cadherins, desmoglein and desmocollin, and was subsequently shown to associate with the classical cadherins E- and N-cadherin. (3,4,7,8,9,15) Plakoglobin’s interaction with E-and N-cadherin lead to its identi cation as the ~80kD protein originally called gamma-catenin. (8) Recent evidence indicates that the classical and desmosomal cadherins actually interact with distinct domains of the plakoglobin protein. (15) Further, the desmosomal cadherins have been shown to regulate the accumulation of plakoglobin in the cell by decreasing its turnover rate. (7) Plakoglobin (g-catenin) is highly homologous to both b-catenin and the product of the Drosophila segment polarity gene armadillo. (8,9,10,11,12) Plakoglobin (g-catenin) and b-catenin contain 13 copies of a 42–44 amino acid motif rst identi ed in the Armadillo protein and referred to as armadillo repeats. (2,10,12) These armadillo repeats not only mediate the interaction between b and g-catenin and the cadherin cytoplasmic domain, but are also responsible for interactions with other cellular proteins.
Catalog #	C2069-60
Applications	Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1ug/ml
	Immuno uorescence: 10ug/ml
	Immunoprecipitation: 2-4ug co-immunoprecipitates plakoglobin in a complex with the desmosomal cadherins, desmoglein (DSg1)and desmocollin (Dsc3a), as well as with E-cadherin.
	Optimal dilutions to be determined by the researcher.
Positive Control	HeLa cells, A431 cells, WI-38 human fibroblasts
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3G62
Host	Mouse
Source	Human
Concentration	0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Fusion protein consisting of the maltose binding protein fused to full length human plakoglobin protein. Epitope has been localized to a site between aa45-114 of the human plakoglobin protein.
Specificity	Recognizes human plakoglobin at ~83kD. Does not appear to crossreact with the related b-catenin protein (~92kD). Species Crossreactivity: mouse
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.