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You are here:Home » Antibodies » Abs to Catenin » Anti -Catenin, gamma (Plakoglobin)

Anti -Catenin, gamma (Plakoglobin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
The catenins (a, b and g) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin. a-Catenin can also bind P-cadherin and N-cadherin. g-Catenin, also known as plakoglobin, is an 82kD protein that binds with a-catenin and N-cadherin.
Catalog #C2069-60A
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:25-50 for 30 min
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlBreast carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide derived from C-terminal of human Catenin-gamma. MW: 82kD
Cellular LocalizationCytoplasmic and cell membrane
SpecificityRecognizes Catenin, gamma.
Species CrossreactivityHuman, Mouse and Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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