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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH

The catenins (a, b and g) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin. a-Catenin can also bind P-cadherin and N-cadherin. g-Catenin, also known as plakoglobin, is an 82kD protein that binds with a-catenin and N-cadherin.
Catalog #	C2069-60A
Applications	Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot: 1ug/ml for 2hrs at RT
	Immunohistochemistry (Formalin/paraffin): 1:25-50 for 30 min
	Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by the researcher.
Positive Control	Breast carcinoma
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide before the addition of glycerol to 40%.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A synthetic peptide derived from C-terminal of human Catenin-gamma. MW: 82kD
Cellular Localization	Cytoplasmic and cell membrane
Specificity	Recognizes Catenin, gamma.
Species Crossreactivity	Human, Mouse and Rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.