USBio Logo

C2085-63J Mouse Anti-Casein Kinase 2 alpha (CKIIa CK2A1, CSNK2A1)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Swiss Prot
P68400
Isotype
IgG1
Clone Number
9H211 (1AD9)
Grade
Purified
Applications
DB IC IHC IP WB
Crossreactivity
Hu Mo Rt
Accession #
NM_001895.3
Shipping Temp
Blue Ice
Storage Temp
-20°C

Unlike most serine/threonine kinases, Casein Kinases (CKs) are not activated by specific stimuli, but are essentially constitutively active. CK1 autophosphorylates, causing inactivation, and protein phosphatases reactivate the kinase. The substrates of CKs are numerous, but their function is less clear, perhaps due to the absence of obvious regulation. CK1 isoforms are thought to be involved in the response to DNA damage, while CK2 levels are elevated in many types of cancer.

Applications
Suitable for use in Western Blot, Dot Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 detects Casein Kinase 2 alpha on 10ug of HeLa cell lysates. Also detects CK-2a in extracts of kidney tissue. Various bands are seen between 40 and 44kD. Using 1ug/ml of C2085-63J, the lower detection limit for the holoenzyme is 500ng. The detection limit for the alpha-subunit is 300ng. Dot Blot: 1ug/ml; The lower detection limit for the holoenzyme is 100ng. The detection limit for the alpha-subunit is 60ng. Immunoprecipitation: Immunoprecipitates a protein with a MW of 44kD, CK-2a subunit. A 26kD protein band, the beta-subunit of the heterotetrameric holoenzyme a2b2, is coprecipitated with the CK-2a subunit. Immunohistochemistry: Paraffin sections Immunocytochemistry (Acetone or methanol fix only): 5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant alpha-subunit of Casein Kinase 2.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes the alpha-subunit of Casein Kinase 2. It binds specifically to the catalytic alpha-subunit of Casein Kinase 2 (CK-2a). Species Crossreactivity: human, mouse and rat.
References
1. Trachana, V., et al., International J. Biochemistry & Cell Biology. 37: 478-492 (2005). 2. Schmidt-Spaniol, l., et al., Hybridoma 11: 53 (1992). 3. Grankowski, N., et al., Eur. J. Biochem. 198: 25 (1991). 4. Laemmli, U.K., Nature 227: 680 (1970). 5. Burnette, W.N., Anal. Biochem. 112: 195 (1981).
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved