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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Casein Kinase 2 Assay Kit, RRRDDDSDDD, BioAssay™ (CK2)

Casein Kinase 2 Assay Kit, RRRDDDSDDD, BioAssay™ (CK2)

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Specifications

The assay kit is designed to measure the phosphotransferase activity of Casein Kinase-2 (CK-2) in immunoprecipitates and column fractions. Crude cell lysates may also be used but detergents or biochemicals contained in the cell lysis buffer may inhibit CK-2 activity. Furthermore, although an inhibitor cocktail is included with the kit, editors may suggest other unknown kinases found in crude lysates are responsible for CK-2 substrate phosphorylation. The assay kit is based on phosphorylation of a specific substrate (CK-2 substrate peptide) using the transfer of the gamma-phosphate of [g-32P]ATP by CK-2 kinase. The phosphorylated substrate is then separated from the residual [g-32P]ATP using P81 phosphocellulose paper and quantitated by using a scintillation counter. The assay is linear for incubation times of up to 30 minutes and incorporation of up to 20% of total ATP. Further incubation or incorporation may not be linear and may therefore not be a true indication of CK-2 activity in the sample extract. The enzyme assay is rapid, convenient and fairly specific for CK-2. Each kit contains sufficient reagents for 200 individual CK-2 assays. detection and quantitation of casein kinase 2 activity from cell extracts.
Catalog #C2085-64
Quantity ~200 assays
Kit ComponentsC2085-64A: Assay Dilution Buffer 2x1ml
C2085-64B: CK2 Substrate Peptide 2x1ml
C2085-64C: PKA Inhibitor Cocktail 2x1ml
C2085-64D: Mg/ATP Cocktail 2x1ml
C2085-64E: p81 pre-labeled squares 200
Storage and StabilityStore at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. 
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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