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You are here:Home » Culture Media » Media-Microbiological » Casman Medium Base (Powder)

Casman Medium Base (Powder)

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Specifications

When supplemented with blood will support growth of many fastidious bacteria including Gardnerella vaginalis.
Catalog #C2086
AppearanceBeige, homogeneous, free flowing powder
SolubilityLight amber to medium amber, trace to slight haze
Solubility (Blood) Red, opaque
pH 7.3±0.2
FormulationEnzymatic Digest of Casein 5g
Enzymatic Digest of Animal Tissue 5g
Yeast Enriched Peptone 10g
Beef Extract 3g
Niacinamide 0.05g
p-Aminobenzoic Acid 0.05g
Dextrose 0.5g
Corn Starch 1g
Sodium Chloride 5g
Agar 13.5g
Total: 43g/L
Cultural ResponseHaemophilus influenza ATCC 3056
Neisseria gonorrhoeae ATCC 43070
Neisseria meningitidis ATCC 13090
Streptococcus pneumoniae ATCC 6305
Streptococcus pyogenes ATCC 19615
DirectionsDissolve 43g in 1L of ddH2O. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Autoclave at 121ºC for 15 minutes. After cooling medium to 50ºC, aseptically add 5% sterile blood and 0.15% sterile water-lysed blood solution (one part blood to three parts water).
Storage and StabilityStore powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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