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Anti -Caspase 1 (Interleukin-1Beta Converting Enzyme, ICE)
Anti -Caspase 1 (Interleukin-1Beta Converting Enzyme, ICE)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP IH IC |
|
| Caspase 1/ICE (Interleukin-1b Converting Enzyme) is expressed as a proenzyme of 45kD in many tissues. Upon stimulus it gets activated by proteolytic cleavage. Active Caspase 1 is a tetramer of two subunits p10 and p20 in 2;2 proportion. Caspase 1 (cystein aspartic-specific protease 1) cleaves its substrates after an aspartate residue. It cleaves Interleukin b into its active form which further exerts its proinflammatory effects. Overexpression of caspase 1 can induce apoptosis in fibroblast which can be inhibited by overexpression of Crm A, a protein from pox virus. | | | Catalog # | C2086-66C | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-4ug/ml | | Immunocytochemistry:10-20ug/ml | | Immunoprecipitation: 4-8ug/ml | | Immunohistochemistry: 1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thermerosal, 30% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa224-242, KTSDSTFLVFMSHGIREG, of human caspase-1. | | Specificity | Recognizes human caspase-1. Detects bands of ~45kD (procaspase-1) and ~20kD (cleavage product) by Western Blot. Species Crossreactivity: mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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