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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 1 (Interleukin-1Beta Converting Enzyme, ICE)

Anti -Caspase 1 (Interleukin-1Beta Converting Enzyme, ICE)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IC
Caspase 1/ICE (Interleukin-1b Converting Enzyme) is expressed as a proenzyme of 45kD in many tissues. Upon stimulus it gets activated by proteolytic cleavage. Active Caspase 1 is a tetramer of two subunits p10 and p20 in 2;2 proportion. Caspase 1 (cystein aspartic-specific protease 1) cleaves its substrates after an aspartate residue. It cleaves Interleukin b into its active form which further exerts its proinflammatory effects. Overexpression of caspase 1 can induce apoptosis in fibroblast which can be inhibited by overexpression of Crm A, a protein from pox virus.
Catalog #C2086-66C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-4ug/ml
Immunocytochemistry:10-20ug/ml
Immunoprecipitation: 4-8ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thermasol, 30% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa 224-242, KTSDSTFLVFMSHGIREG, of human caspase-1.
SpecificityRecognizes human caspase-1. Detects bands of ~45kD (procaspase-1) and ~20kD (cleavage product) by Western Blot. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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