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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 1 (Interleukin-1Beta Converting Enzyme, ICE)

Anti -Caspase 1 (Interleukin-1Beta Converting Enzyme, ICE)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B FC
Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45kD.
Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1 beta from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL- beta processing and release.
Catalog #C2086-66G
ApplicationsSuitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D213
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenP20 fragment of mouse caspase-1 and a synthetic peptide corresponding to aa206-220, TALEMVKEVKEFAAC of mouse caspase-1.
SpecificityRecognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kD by Western Blot. Does not cross-react with mouse caspase-11 or -12.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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