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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 1 (Interleukin-1Beta Converting Enzyme, ICE)

Anti -Caspase 1 (Interleukin-1Beta Converting Enzyme, ICE)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IC
Caspase 1 (ICE, interleukin-1beta converting enzyme) is a member of the ICE family of cysteine proteases. Caspase 1 exists in many cells as an inactive 45kD proenzyme and is cleaved into 20kD and 10kD subunits which form the active caspase 1. Casapse 1 cleaves the inactive form of interleukin-1beta (IL-1beta) to generate the active from of this pro-inflammatory cytokine. Like the other caspases, Caspase 1 plays a role in apoptosis. Overexpression of Caspase 1 in cells induced apoptosis, and Caspase 1-mediated apoptosis can be blocked by CrmA, a selective inhibitor of several caspases.
Catalog #C2086-68J
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.5-2ug/ml
Immunoprecipitation: ~5ug/0.5mg of cell protein
Immunohistochemistry: 10ug/ml
Optimal dilutions to be determined by researcher.
Recommended Positive ControlHuman HL-60 cells and mouse ABE 8 1/2 cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 01.M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa129-149, PEHKTSDSTFLVFMSHGIREG, from human Caspase 1, conjugated to KLH.
SpecificityRecognizes the proteolytic fragment (20kD) and the full length proenzyme (45kD) of human Caspase 1. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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