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You are here:Home » Antibodies » Antibodies-Apoptosis, Caspase » Anti -Caspase 2 (ICH1, Nedd-2)

Anti -Caspase 2 (ICH1, Nedd-2)


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Clone Host Grade Applications
Monoclonal Mouse Ascites B
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-2 has a positive and a negative role in the regulation of the apoptotic process. Overexpression of the full caspase-2 (Nedd2/Ich1 ) mRNA in some cell types results in apoptosis, whereas overexpression of an alternative caspase-2 splice variant suppresses apoptosis induced by serum withdrawal. Caspase-2 may be activated in vitro by caspase-1, caspase-3 and the neutral serine protease granzyme B which is stored in the specialized lytic granules of cytotoxic lymphocytes.
Catalog #C2086-72C
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlHL60 cells
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No4H39
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
ImmunogenA peptide corresponding to a portion of human Caspase-2
SpecificityRecognizes human caspase-2 long form (~48kD). Species Sequence Homology: Reactivity is predicted with rat and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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