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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 2 (ICH-1, Nedd2)

Anti -Caspase 2 (ICH-1, Nedd2)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B
Caspase-2 (Nedd2/ICH-1) is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved at Asp316, producing a 14kD fragment and a 32kD prodomain/large subunit. Subsequent processing at Asp152 and Asp330 produces an 18kD large subunit and a 12kD small fragment (1). Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD (2). This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events (3, 4). In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase- 3 activation, extending a possible role for caspase- 2 as a parallel effector caspase (5).
Catalog #C2086-72D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No4i13
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
PurityAscites
ImmunogenSynthetic peptide corresponding to the carboxy-terminal portion of human caspase-2.
SpecificityRecognizes endogenous levels of human procaspase-2 at ~48kD as well as its 14 and 12kD cleaved fragments.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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