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You are here:Home » Kits and Assays » Kits for Apoptosis, BioAssay™ » Caspase 2 (ICH1, Nedd2), Apoptosis Detection, Colorimetric BioAssay™ Kit

Caspase 2 (ICH1, Nedd2), Apoptosis Detection, Colorimetric BioAssay™ Kit

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Specifications

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2/ICH-1 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity.
Catalog #C2086-74
Components 25 assays 100 assays
Cell Lysis Buffer 25ml 100ml
2X Reaction Buffer 2ml 4 x 2ml
VDVAD-pNA Substrate (4mM) 125ul 500ul
DTT (1M) 100ul 400ul
Dilution Buffer 25ml 100ml
StabilityStore kit at -20°C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening). All reagents are stable for 1 year under proper storage conditions.
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3. Caspase-2, -3, -6, -8, -9 Fluorometric and Colorimetric Assay Kits and Reagents
4. Caspase Inhibitors and Inhibitor Set
5. Active Recombinant Human Caspases
6. Quick Apoptotic DNA Ladder Kits and In Situ Detection Kits
7. Apoptosis Inducers
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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