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Caspase 2 (ICH1, Nedd2), Apoptosis Detection, Colorimetric BioAssay™ Kit
Caspase 2 (ICH1, Nedd2), Apoptosis Detection, Colorimetric BioAssay™ Kit
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| Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2/ICH-1 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity. | | | Catalog # | C2086-74 | | Components | 25 assays 100 assays | | Cell Lysis Buffer 25ml 100ml | | 2X Reaction Buffer 2ml 4 x 2ml | | VDVAD-pNA Substrate (4mM) 125ul 500ul | | DTT (1M) 100ul 400ul | | Dilution Buffer 25ml 100ml | | Stability | Store kit at -20°C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening). All reagents are stable for 1 year under proper storage conditions. | | | | Related Products | | | 1. Annexin V-FITC, -Cy3, and -EGFP Apoptosis Detection Kits and Reagents | | 2. Annexin V-Biotin, and Unlabelled Annexin V | | 3. Caspase-2, -3, -6, -8, -9 Fluorometric and Colorimetric Assay Kits and Reagents | | 4. Caspase Inhibitors and Inhibitor Set | | 5. Active Recombinant Human Caspases | | 6. Quick Apoptotic DNA Ladder Kits and In Situ Detection Kits | | 7. Apoptosis Inducers | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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