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Caspase 2, Apoptosis Detection, Fluorometric BioAssay™ Kit (ICH1, Nedd2)
Caspase 2, Apoptosis Detection, Fluorometric BioAssay™ Kit (ICH1, Nedd2)
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| Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2/ICH-1 Fluorometric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (lmax 400nm); upon cleavage of the substrate by ICH-1 or related caspases, free AFC emits a yellow-green fluorescence (lmax 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity. | | | Catalog # | C2086-75 | | Components | 25 assays 100 assays | | Cell Lysis Buffer 25ml 100ml | | 2X Reaction Buffer 2ml 4 x 2ml | | VDVAD-AFC Substrate (1mM) 125ul 500ul | | DTT (1M) 100ul 400ul | | Stability | Store kit at -20°C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening). All reagents are stable for 1 year under proper storage conditions. | | Related Products | | | 1. Annexin V-FITC, -Cy3, and -EGFP Apoptosis Detection Kits and Reagents | | 2. Annexin V-Biotin, and Unlabelled Annexin V | | 3. Caspase -3, -6, -8, -9 Fluorometric Protease Assay Kits and Reagents | | 4. Caspase-2, -3, -6, -8, -9 Colorimetric Protease Assay Kits and Reagents | | 5. Active Recombinant Human Caspase-3/CPP32 | | 6. Quick Apoptotic DNA Ladder Detection Kits | | 7. In Situ DNA Fragmentation Detection Kits and Reagents | | 8. Apoptosis Inducers | | 9. Apoptosis Inhibitors | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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