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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

Anti -Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B
Catalog #C2087-01G
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Positive Control Staurosporine (2mM) treated HeLa, Jurkat, Raw, NIH3T3
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No8J396
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, containing 0.2% gelatin and 0.05% sodium azide.
PurityAscites
ImmunogenFull-length recombinant human caspase-3 protein
SpecificityRecognizes human Caspase 3. Detects both pro caspase-3 (~32kD) and the large subunit of the active/cleaved form (~14-21kD) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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