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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

Anti -Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP
Caspase-3 is one of the key executioners of apoptosis, being responsible either partially or totally for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADPribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 subunits. Cleavage of caspase-3 requires aspartic acid at the P1 position.
Catalog #C2087-03A
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG
Clone No9j114
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to amino-terminal residues adjacent to (Asp175) in human Caspase 3.
SpecificityRecognizes endogenous levels of full length (35kD) and large fragment (17/19kD) of human Caspase 3 resulting from cleavage at (Asp175). Species Crossreactivity: mouse, rat and monkey
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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