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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

Anti -Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase 3 is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP). Caspase 3 is a cytosolic protein found in cells as an inactive 32kD proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis.
Catalog #C2087-04E
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000-1:10,000
Immunoprecipitation: 1:10
Immunohistochemistry (Paraffin): 1:25-1:50
Immunocytochemistry: 1:25
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k75
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues from the p17 subunit of human Caspase 3.
SpecificityRecognizes human Caspase 3. Recognizes both the pro-form (35kD) and the p17 cleaved-form of Caspase 3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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