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Caspase 3 Apoptosis Detection, Fluorometric BioAssay™ Kit (CPP-32, Apoptain, Yama, SCA-1)
Caspase 3 Apoptosis Detection, Fluorometric BioAssay™ Kit (CPP-32, Apoptain, Yama, SCA-1)
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| Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3 Fluorometric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (lmax 400nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (lmax 505nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity. | | | Catalog # | C2087-10 | | Kit Components | 25 assays 100 assays | | C2087-10A Cell Lysis Buffer 25ml 100ml | | C2087-10B 2X Reaction Buffer 2ml 4 x 2ml | | C2087-10C DEVD-AFC Substrate (1mM) 125ul 500ul | | C2087-10D DTT (1M) 100ul 400ul | | Storage and Stability | Store kit at -20°C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening). All reagents are stable for 6 months under proper storage conditions. Protect DEVD-AFC from light. | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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