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You are here:Home » Kits and Assays » Kits for Apoptosis, BioAssay™ » Caspase 3 Apoptosis Detection, Fluorometric BioAssay™ Kit (CPP-32, Apoptain, Yama, SCA-1)

Caspase 3 Apoptosis Detection, Fluorometric BioAssay™ Kit (CPP-32, Apoptain, Yama, SCA-1)

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Specifications

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3 Fluorometric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (lmax 400nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (lmax 505nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
Catalog #C2087-10
Kit Components 25 assays 100 assays
C2087-10A Cell Lysis Buffer 25ml 100ml
C2087-10B 2X Reaction Buffer 2ml 4 x 2ml
C2087-10C DEVD-AFC Substrate (1mM) 125ul 500ul
C2087-10D DTT (1M) 100ul 400ul
Storage and StabilityStore kit at -20°C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening). All reagents are stable for 6 months under proper storage conditions. Protect DEVD-AFC from light.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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