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You are here:Home » Molecular Biology » MB-Apoptosis » Caspase 3 Fluorometric Substrate, DEVD (CPP-32, Apoptain, Yama, SCA-1)

Caspase 3 Fluorometric Substrate, DEVD (CPP-32, Apoptain, Yama, SCA-1)

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Specifications

Fluorometric substrate for Caspase-3.
N-acetyl-Asp-Glu-Val-Asp-AFC; 7-amino-4-trifluoromethyl coumarin
Catalog #C2087-11
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Used in the BioAssay™ Caspase-3 Apoptosis Detection Kit (Cat.# C2087-10), providing a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7uM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC (7-amino-4-trifluoromethyl coumarin) at Ex 400nm and Em 505nm, using a fluorometer or a multi-well fluorescence plate reader. Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increase in the yellow-green fluorescence is proportional to the increase in caspase activity.Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
Storage and StabilityStore at -20°C, protected from light.
6 months under proper storage conditions
Molecular FormulaC H F N O
Molecular Weight729.0
Purity98% (HPLC)
FormSupplied as a liquid in 1mM DMSO.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesN-acetyl-Asp-Glu-Val-Asp-AFC; 7-amino-4-trifluoromethyl coumarin


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