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Caspase 3 Fluorometric Substrate, DEVD (CPP-32, Apoptain, Yama, SCA-1)
Caspase 3 Fluorometric Substrate, DEVD (CPP-32, Apoptain, Yama, SCA-1)
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| Fluorometric substrate for Caspase-3. | | | N-acetyl-Asp-Glu-Val-Asp-AFC; 7-amino-4-trifluoromethyl coumarin | | | Catalog # | C2087-11 | | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Used in the BioAssay™ Caspase-3 Apoptosis Detection Kit (Cat.# C2087-10), providing a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. | | Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7uM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC (7-amino-4-trifluoromethyl coumarin) at Ex 400nm and Em 505nm, using a fluorometer or a multi-well fluorescence plate reader. Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increase in the yellow-green fluorescence is proportional to the increase in caspase activity.Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. | | Storage and Stability | Store at -20°C, protected from light. | | 6 months under proper storage conditions | | Molecular Formula | C H F N O | | Molecular Weight | 729.0 | | Purity | 98% (HPLC) | | Form | Supplied as a liquid in 1mM DMSO. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | N-acetyl-Asp-Glu-Val-Asp-AFC; 7-amino-4-trifluoromethyl coumarin |
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