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You are here:Home » Kits and Assays » Kits-Apoptosis, BioAssay™ » Caspase 3 BioAssay™ ELISA Kit (CPP-32, Apoptain, Yama, SCA-1)

Caspase 3 BioAssay™ ELISA Kit (CPP-32, Apoptain, Yama, SCA-1)


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This Caspase 3 ELISA Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex. = 400nm and Em. = 505nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected.
Catalog #C2087-12
Kit ComponentsC2087-12A: Cell Lysis Buffer 1x25ml *
C2087-12B: Coating Buffer 1x10ml *
C2087-12C: Antibody, 20x 1x500ul
C2087-12D: Blocking Buffer 1x15ml *
C2087-12E: Incubation Buffer 1x100ml *
C2087-12F: DTT (1M) 1x400ul
C2087-12G: DEVD-AFC Substrate 1x500ul
C2087-12H: Positive Control 1x10units
C2087-12J: Microtiter Plate 1x12x8wells
Storage and StabilityStore all components at -20°C. After thawing, store Cell Lysis Buffer, Coating Buffer, Blocking Buffer and Incubation Buffer at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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