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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

Anti -Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Caspase-3 is one of the key executioners of apoptosis, being responsible either partially or totally for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADPribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 subunits. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).
Catalog #C2087-16
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunohistochemistry (Paraffin): 1:200
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to residues surrounding the cleavage site and the amino terminus of the large fragment of human caspase-3 (KLH).
SpecificityRecognizes human caspase-3 (35kD) and the large fragment of caspase-3 resulting from cleavage (17, 19kD). Species Crossreactivity: mouse, rat and monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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