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You are here:Home » Kits and Assays » Kits for Apoptosis, BioAssay™ » Caspase 3 Apoptosis Detection, Colorimetric BioAssay™ Kit (CPP-32, Apoptain, Yama, SCA-1)

Caspase 3 Apoptosis Detection, Colorimetric BioAssay™ Kit (CPP-32, Apoptain, Yama, SCA-1)

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Specifications

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
Catalog #C2087-20
Kit Components (100 assays)C2087-20A: Cell Lysis Buffer
C2087-20B: 2X Reaction Buffer
C2087-20C: DEVD-pNA (4mM)
C2087-20D: DTT (1M)
C2087-20E: Dilution Buffer
Storage and StabilityStore kit at -20°C before use. After opening, store C2087-20A, Cell Lysis Buffer, C2087-20B, 2X Reaction Buffer and C2087-20E, Dilution Buffer at 4°C. Stable for 6 months.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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