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Caspase 3 Apoptosis Detection, Colorimetric BioAssay™ Kit (CPP-32, Apoptain, Yama, SCA-1)
Caspase 3 Apoptosis Detection, Colorimetric BioAssay™ Kit (CPP-32, Apoptain, Yama, SCA-1)
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| Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity. | | | Catalog # | C2087-20 | | Kit Components (100 assays) | C2087-20A: Cell Lysis Buffer | | C2087-20B: 2X Reaction Buffer | | C2087-20C: DEVD-pNA (4mM) | | C2087-20D: DTT (1M) | | C2087-20E: Dilution Buffer | | Storage and Stability | Store kit at -20°C before use. After opening, store C2087-20A, Cell Lysis Buffer, C2087-20B, 2X Reaction Buffer and C2087-20E, Dilution Buffer at 4°C. Stable for 6 months. | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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