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You are here:Home » Kits and Assays » Kits-Apoptosis, BioAssay™ » Caspase 3, Cleaved (Asp175), Apoptosis Marker,) Western Detection Kit, BioAssay™ (CPP-32, Apoptain, Yama, SCA-1)

Caspase 3, Cleaved (Asp175), Apoptosis Marker,) Western Detection Kit, BioAssay™
(CPP-32, Apoptain, Yama, SCA-1)


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Synthetic peptide(KLH coupled) corresponding to N-terminal residues adjacent to Asp175 in human caspase-3. Synthetic peptide (KLH coupled) corresponding to residues surrounding the cleavage site and the amino terminus of the large fragment of human caspase-3.
Catalog #C2087-20K
Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases. Caspase-3 Antibody detects endogenous levels of full length caspase-3 (35kD) and the large fragment of caspase-3 resulting from cleavage (17kD).
Caspase-3 is one of the key executioners of apoptosis, being responsible either partially or totally for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 subunits. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). Apoptosis Marker Cleaved Caspase-3 (Asp175) Western Detection Kit contains enough primary caspase-3 and cleaved caspase-3 antibodies, and secondary anti-rabbit and anti-biotin antibodies to perform 10 Western mini-blots. The kit also comes with biotinylated molecular weight markers, a set of control cell lysates and reagent. The kit offers an efficient way of detecting caspase-3 processing and activation by Western immunoblotting.
ApplicationsSuitable for use in Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Form Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Purity Purified by protein A and peptide affinity chromatography.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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