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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 3 (BSA & Azide Free) (CPP-32, Apoptain, Yama, SCA-1)

Anti -Caspase 3 (BSA & Azide Free) (CPP-32, Apoptain, Yama, SCA-1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH
Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kD) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kD and 12kD, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to a variety of apoptotic inducers including Fas mediated apoptosis.
Catalog #C2087-22C1X
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoprecipitation (Native and denatured): 2ug/mg protein lysate (Use Protein A).
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 min at RT; 3ug/ml is better. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution to be determined by the researcher.
Positive Control Jurkat cells, tonsil and appendix.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3F47
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C2087-22C1
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant full length human Caspase 3 protein. Cellular Localization: Predominantly cytoplasmic with some nuclear staining.
SpecificityRecognizes Caspase 3 (CPP32) at 32kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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