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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 3 (BSA & Azide Free) (CPP-32, Apoptain, Yama, SCA-1)

Anti -Caspase 3 (BSA & Azide Free) (CPP-32, Apoptain, Yama, SCA-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kD) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kD and 12kD, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis.
Catalog #C2087-22C4X
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 2.5-5ug/ml for 2hrs at RT
Immunoprecipitation (Denatured): 10ug/mg protein lysate (Use Protein A).
Immunohistochemistry (Formalin/paraffin): 5-10ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat cells and Tonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No stabilzing proteins and no preservatives added. Also available with BSA and azide. See C2087-22C4.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant full length human caspase 3 protein. Cellular Localization: Predominantly cytoplasmic with some nuclear staining.
SpecificityRecognizes human Caspase 3 (CPP32) at 32kD. Species Crossreactivity: Mouse, rat, monkey, rabbit, hamster, bovine, sheep, porcine and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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