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You are here:Home » Kits and Assays » Kits for Apoptosis, BioAssay™ » Caspase 6 (McH2), Apoptosis Detection, Fluorometric BioAssay™ Kit

Caspase 6 (McH2), Apoptosis Detection, Fluorometric BioAssay™ Kit

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Specifications

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Mch2/Caspase-6 Fluorometric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on detection of cleavage of substrate VEID-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VEID-AFC emits blue light (lmax 400nm); upon cleavage of the substrate by Mch2 or related caspases, free AFC emits a yellow-green fluorescence (lmax 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Mch2 activity.
Catalog #C2087-26
Components 25 assays 100 assays
Cell Lysis Buffer 25ml 100ml
2X Reaction Buffer 2ml 4 x 2ml
VEID-AFC Substrate (4mM) 125ul 500ul
DTT (1M) 100ul 400ul
StabilityStore kit at -20°C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening). All reagents are stable for 1 year under proper storage conditions.
Related Products
1. Annexin V-FITC, -Cy3, and -EGFP Apoptosis Detection Kits and Reagents
2. Annexin V-Biotin, and Unlabelled Annexin V
3. Caspase-2, -3, -6, -8, and -9 Fluorometric and Colorimetric Assay Kits
4. Active Recombinant Human Caspases
5. Quick Apoptotic DNA Ladder Detection Kits
6. In Situ DNA Fragmentation Detection Kits and Reagents
7. Apoptosis Inducers
8. Apoptosis Inhibitors
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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